Exploration and clinical outcomes of Half-ICSI using sibling oocytes from patients in vitro reproductive technology

【Objective】To determine an optimal insemination technique for patients suspected of high risk of fertilization failure and undergoing assisted reproduction treatment. 【Methods】One hundred and seven couples were treated by conventional in vitro fertilization(IVF) and intracytoplasmic sperm injection(ICSI) in one cycle(half-ICSI) by dividing the sibling oocytes in halves from January 2009 to December 2011. The clinical and laboratory data were analyzed.【Results】In the study, the fertilization rate of ICSI(88.3%) was significantly higher than that of IVF(52.1%)(P 0.01), and the rates of complete fertilization failure were(24.3%) and low fertilization(6.5%) of IVF than those of ICSI(0 and 0%), the level one of quality embryos rate of IVF was significantly lower than that of ICSI group(P 0.05). No significant differences were observed in the rates of cleavage, implantation and pregnancy between the two groups(P 0.05).【Conclusion】There are indications and necessity of half-ICSI in one cycle in order to improve rate of fertilization, achieve more high quality embryos for transferring and improving the rate of pregnancy for patients with high risk of fertilization failure. For the cycles with fertilization both in conventional IVF and ICSI, the option of conventional IVF embryos transplantation may reduce the risks of genetic and congenital defect of the offspring.