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Molecular Cloning of a Gene Suppressed by Heat Shock and Its Expression Characteristics.

Jianxin Dai, Yilun Wang, Fengjuan Zhou, Deru Lu, Yujian Liu, Renbao Xu

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Abstract

On the base of a cDNA fragment cloned from a human osteosarcoma cell line (HOS-8603) which was taken as the model of heat shock by DDRT-PCR, the cDNA library was screened by the probe made from this fragment, and the colony containing full length cDNA of this cDNA fragment (HSSG-1) was identified. Sequencing data indicated that this cDNA consisted of 1 456 bp, coding for 276 amino acids. Computer aided homology analysis did not find the same sequence published. The result of RNA dot hybridization suggested that HSSG-1 was expressing in many kinds of organs and cells, and the down regulating of its expression level caused by heat shock was general. Therefore, this may be a gene that is suppressed by heat shock.

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What this paper is about

On the base of a cDNA fragment cloned from a human osteosarcoma cell line (HOS-8603) which was taken as the model of heat shock by DDRT-PCR, the cDNA library was screened by the probe made from this fragment, and the colony containing full length cDNA of this cDNA fragment (HSSG-1) was identified. Sequencing data indicated that this cDNA consisted of 1 456 bp, coding for 276 amino acids. Computer aided homology analysis did not find the same sequence published. The result of RNA dot hybridization suggested that HSSG-1 was expressing in many kinds of organs and cells, and the down regulating of its expression level caused by heat shock was general. Therefore, this may be a gene that is suppressed by heat shock.

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Available abstract

On the base of a cDNA fragment cloned from a human osteosarcoma cell line (HOS-8603) which was taken as the model of heat shock by DDRT-PCR, the cDNA library was screened by the probe made from this fragment, and the colony containing full length cDNA of this cDNA fragment (HSSG-1) was identified. Sequencing data indicated that this cDNA consisted of 1 456 bp, coding for 276 amino acids. Computer aided homology analysis did not find the same sequence published. The result of RNA dot hybridization suggested that HSSG-1 was expressing in many kinds of organs and cells, and the down regulating of its expression level caused by heat shock was general. Therefore, this may be a gene that is suppressed by heat shock.

Key concepts: Complementary DNA, cDNA library, Molecular biology, Gene, Heat shock protein, Molecular cloning, Cloning (programming), Biology

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