Identification and characterization of interspecific hybrids from Brassica napus(Eru CMS)×Brassica oleracea

Hybrids from Brassica napus×Brassica oleracea were obtained by embryo rescue techniques,which is an effective approach for increasing the resources of male sterility of Brassica oleracea.Both the DNA content and chromosome number of hybrids were the mid-parent values.Characteristics of the hybrid were investigated.The results showed that hybrids were morphologically similar to parents during the growing periods,while inclined to the female parent during flowering.But hybrids had strong heterosis of growth.The leaf structure,chloroplast number in stomata guard cell,the floral organ,and the early flowering periods of hybrids were mid-parent,except the mutated leaf structure of mosaic hybrid.Hybrids had their own unique peroxidase isozymes bands.Test of the pollen viability showed that hybrids bore only non-viability pollens with 100% sterility.Backcross generation could be obtained only through embryo rescue techniques to overcome barriers of hybridization.