THE EFFECT OF siRNA ON THE EXPRESSION OF HUMAN GASTRIC CANCER CELL LINE SGC-7901

ObjectiveTo evaluate the gene expression of VEGF by RNA interference in human gastric cancer.Methods(siRNAs) targeting VEGF mRNA was obtained by in vitro transcription,which was transfected into human gastric cancer cell lines SGC-7901 with Lipofectamine 2000.The non-transfected cells,cells treated with Lipofectimine and cells treated with SCR were taken as controls.MTT was used to detect the inhibitive rate of cell growth.Flow cytometry was used to detect the changes of cycling of the cell.The inhibitive effect of siRNA on mRNA level was detected by RT-PCR,the secretion of VEGF protein in the supernatant by ELISA.ResultsThe small interfering RNA(siRNA) targeting human VEGF effectively inhibited the proliferation of gastric cancer SGC-7901.It affected the distribution of cell cycle,increased cell population in G_0/G_1 phase and decreased it in S phase.The expression of VEGF mRNA was significantly inhibited in SGC-7901;otherwise,VEGF protein notably decreased,but the effects did not appear in control scramble siRNA.ConclusionThe level of VEGF gene expression of SGC-7901 cells can be declined by transfected with siRNA.