Study on Relations of Apoptosis Induced by Tamoxifen and Chemoresistance with Survivin in Breast Cancer

Objective:To study tamoxifen(TAM) inducing growth arrest and apoptosis and explore chemoresistance with survivin in breast cancer cell line.Method:Detect apoptosis by flow cytometry and measure cell proliferation activity by MTT assary in breast cancer. The cultured MCF-7 cells was treated with TAM.Then,the expression of survivin mRNA and protein by MCF-7 cells was evaluated by semi-quantitative reverse-transcription polymerase chain reaction (RT-PCR) and western blot analysis.Results:When tamoxifen was 1μmol,promoting apoptosis,inhibiting proliferation activity and survivin mRNA protein expression singnificantly decreasring was detected in the human breast cancer cell line.Conclusion:Apoptosis induced by tamoxifen in breast cancer was associated with survivin that plays important role in regulation of cell cycle.survivin was related to the apoptosis and proliferation of carcinoma cells and drug resistance.Apoptosis induced by tamoxifen in breast cancer has been associated with survivin.survivin could be a target in gene therapy.