Effect of Salvia miltiorrhiza on the Expression of on MMP-2,TIMP-1,TGF-β_1 and IV-C of Rats with Dibetic Nephropathy

Objective To study the effect of Salvia miltiorrhiza on the expression of MMP-2,TIMP-1,TGF-β1 and IV-C in Kidney of rats with diabetes and its relationship with diabetic nephropathy(DN).Methods SD rats were randomly divided into normal control group,model group,treatment group and observation group,with 10 rats in each group.The type II diabetic rat model was constructed by employing high glucose and lipid,and low dose STZ,and the animals were sacrificed at the end of 16th week.Immunohistochemistry was adopted to detect the expression of MMP-2,TIMP-1,TGF-β1 and IV-C in renal tissues,meanwhile,serum glucose,urine protein and renal function were also evaluated.Results Compared with the treatment group and observation group,the expression of TGF-β1,TIMP-1 and IV-C significantly decreased(P0.05),but MMP-2 expression increased(P0.05).At the same time,the changes of MMP-2 and IV-C expression were significantly correlated with urine protein excretion.Conclusion The expression of TIMP-1,TGF-β1 and IV-C are related to DN,Salvia miltiorrhiza has protective effect on DN through affecting the changes of above mentioned indexes.