Effect of fas gene transfection on biological characteristics of tongue squamous cell carcinoma cell line Tca8113 cells

Objective:To investigate effect of fatty acid synthase(fas)gene transfection on biological characteristics of tongue squamous cell carcinoma cell line Tca8113 and its molecular mechanism. Methods: Plasmid including fas gene was transfected into Tca8113 cells by lipofectamine kit. Fas protein expression in tumor cells was detected by flow cytometry(FCM). Cell's response to 5-Fu was assayed by MTT technique. Apoptosis in tumor cells was examined by TUNEL. And PBMC killing activity against carcinoma cells was evaluated by H33342 release procedure. Results:fas gene transfection regulated fas protein expression intensity from 35.01±5.26 in untrasfected cells up to 55.40±7.31 in transfected cells. Under the same concentration of 5-Fu and anti-fas antibody, cell death and apoptosis index (AI) increased in fas transfection cells. tatistically, The difference of cell killing rate and AI between untransfected(16.88%±1.46% )and transfected cells(27.12%±2.35%)were significant(P0.01). PBMC killing activity on untransfected and transfected Tca8113 cells were 23.92%±2.38% and 51.22%±4.61% respectively and also had significant difference(P0.01). Conclusions: Fas gene transfection enhances killing ability of chemotherapy drug and facilitates immunological cell against oral squamous cell carcinoma cells.