2007•Yiyao daobaoRequires access
Inhibitory Effect of Aconitum Coadministration with Ampelopsis on Cytochrome P_(450) in Rat Livers
Yue Gao
Open publisher page 0 citations
Abstract
Objective To study the effect of Aconitum coadministration with ampelopsis on the enzyme activity,protein expression and mRNA level of cytochrome P450 isoenzymes in rat liver. Methods CYP1A2 and CYP2E1 activities were quantitated by high performance liquid chromatographic(HPLC) assay;CYP3A1/2 activity was quantitated by UV chromatography.The protein expressions of CYP1A2,CYP2E1,CYP3A1,and CYP3A2 were detected by Western blot.The mRNA levels of CYP1A2,CYP2E1,CYP3A1,and CYP3A2 were detected by semi-quantitative reverse transcriptase-polymerase chain reaction(RT-PCR). Results Aconitum coadministration with ampelopsis obviously inhibited the activities of CYP1A2,CYP2E1 and CYP3A1/2;Western Blot showed a decreased protein expression of CYP2E1,and CYP3A2,and an increased protein expression of CYP1A2 and CYP3A1.RT-PCR showed an increased mRNA level of CYP1A2,CYP2E1,CYP3A1,and CYP3A2 as compared with the control group. Conclusion Aconitum coadministration with ampelopsis has an inhibitory effect on the enzymes activity of CYP1A2,CYP2E1,and CYP3A1/2. The enzymes activity inhibitory effect of CYP2E1 may be related to the decrease of its protein expression,but not CYP1A2,CYP3A1,and CYP3A2.