The study of the effect mechanism of berberine on anti-tumor angiogensis

Objective:To study the effect of berberine on cell proliferation, cell cycle and cell apoptosis in bFGF activated HUVEC. To investigate the mechanism of inhibitory tumor angiogenesis.Methods:MTT assay was used to determine the cell proliferation effect by berberine. The change of cell morphology and cell cycle was detected by laser scanning confocal microscope and flow cytometry, respectively. Flow cytometry was applied to detected the cell apoptosis rate.Results:The proliferation of HUVEC was inhibited by berberine in a dose-dependent manner. The cell cycle were arrested in G0-G1.The cell nuclear condensation and nuclear cataclasm were found under laser scanning confocal microscope. Simultaneity increased intracellular calcium ion was observed after drug treatment. Berberine induced activated HUVEC apoptosis.Conclusion:Berberine could inhibit tumor angiogenesis by arresting activated HUVEC in G0-G1, proliferation and apoptosis of tumor.