Effects of Applying Organic Fertilizer on Morphology and Structure of Chloroplast and Exudates of Flue-cured Tobacco Trichomes

Taking the flue-cured tobacco K326 potted as experiment materials,under the different fertilizer types(organic fertilizer and chemical fertilizer),we studied the morphology and structure development and the differences of trichome exudates of flue-cured tobacco.The observation by fluorescence microscopy indicated that the fluorescence intensity of chlorophyll was stronger in organic manure treatment after transplanted 60 days.The observation by transmission electron microscope indicated that the chloroplast structure with well developed thylakoid grana lamellae was intact in trichome head cells and in which there were a large amount of high electronic osmiophilic globules.In chemical fertilizer treatment,the osmiophilic globules were smaller amount and volume.GC / MS analysis on the tobacco leaf extract under different fertilizer conditions: the content of duvatriene diol of the mostly secretion of tobacco trichome,under organic fertilizer was higher than that under the chemical fertilizer.The correlation analysis showed that the exudates content was signilicant between the different treatments.It indicated that fertilizer had significant implications on secretion activities of tobacco trichomes.