Effect of hypoxia on the expression of hypoxia inducible factor 1α,P53 and vascular endothelial growth factor in lung of mice

Objective:To investigate the expression of hypoxia inducible factor-1α,p53 and VEGF in lung of mice during hypoxia and its relationship with angiogenesis.Method:The mice were divided into two group(hypoxia group and control group).The hypoxia group mice were placed in a hypoxia chamber with 10%,7%,or 5% O2 respectively for 3,6,9 days. The immunohistochemical staining technique was used to examine HIF-1α,P53,VEGF and MVD. Result: HIF-1αproteins did not express,and p53 and VEGF proteins expressed weakly in control group mice. Compared with the controls,the expression of HIF-1α,p53 and VEGF increased markedly. There was significant difference between the hypoxia group and the control group. The positive relationships were found between the expression of HIF-1α, p53, VEGF and MVD. Conclusion:HIF-1α/VEGF pathway may play an important role in angiogenesis of hypoxia in the lung of mice. Loss of p53 function may faciliate angiogenesis via HIF-1α/VEGF pathway.