Construction of an Eukaryotic Expression Plasmid for NcSRS2 Gene of Neospora caninum

Based on the NcSRS2 gene sequence of Neospora caninum, a pair of primers containing Kozak sequence, Pstand Xbaenzyme digestion sites were designed .Using the plasmid P43 which contains NcSRS2 gene as template,the ORF region of NcSRS2 gene was amplified by polymerase chain reaction (PCR), then the PCR product was digested with Pstand Xba,and the eukaryotic expression vector pcDNA3.1(+)was also done ,then the gene was cloned into the vector.Finally a recombinant plasmid named pcNcSRS2 was obtained, and was identified by PCR, restriction enzyme analysis and sequencing.