Effects of medicated serum with Xintongshu on apoptosis of cardiac myocyte and apoptosis-related gene Bcl-2 and Bax expression after hypoxia-reperfusion in neonatal rats

Objective To study the effects of medicated serum with Xintongshu on apopto-sis of cardiac myocyte and apoptosisrelated gene Bcl-2、Bax expression after hypoxia-reperfusion(H/R) in neonatal rats and investigste its protective mechanism.Methods The cardiomyocyte H/R models of neonatal rats were randomly divided into four groups namely normal serum control group,H/R group,AG490 group and serum containing Xintongshu group.Luodanming B stain technology was used to observe the morphology of cardiomyocyte,AO/EB double fluores-cent staining to detect the apoptosis of cardiomyocyte,and RT-PCR to test the expression of mRNA of the gene-related apoptosis Bcl-2 and Bax。 Results Compared with control group,the mRNA expression of Bcl-2 in H/R group decreased but that of Bax and Caspase-3 increased(P 0.01);Compared with H/R group,the mRNA expression of Bcl-2 in medicated serum group and AG 490 group increased but that of Bax decreased(P0.01) Compared with AG 490 group,the mRNA expression of Bcl-2 in medicated serum group increased but that of Bax decreased(P0.05).Conclusion Xintongshu can protect cardiac myocyte of neonatal rat,The mechanism may be related to down-regulate bid pro-apoptosis gene bid bax and up-regulate the apoptosis gene repressor Bcl-2 and Bcl-2/Bax,and restrain the apoptosis of cardiomyocyte.