Nephrotoxicity of gemcitabine on human breast cancer cell MCF-7 and humanermbryonic kidney cell 293 invitro

OBJECTIVE To demonstrate the antiproliferative effect of gemcitabine on human ermbryonic kidney cell 293 and human breast cancer cell MCF-7 in vitro.METHODS The inhibiting rate of gemcitabine on human ermbryonic kidney cell 293 and human breast cancer cell MCF-7 was detected by MTT assay.Their IC50 values for the effect of gemcitabine were compared.The cell growth curve was designed.The contents of SOD,MDA and LDH in cultural supernate were measured by ultraviolet spectrophotometry.RESULTS Gemcitabine significantly inhibited the proliferation of human breast cancer cell MCF-7 in vitro.It also inhibited the proliferation of human ermbryonic kidney cell 293.Their chemotherapeutic index were 26.907(24 h),15.307(48 h) and 10.264(72 h).CONCLUSION Gemcitabine is cytotoxic in human breast cancer cell MCF-7,and inhibits the proliferation of human ermbryonic kidney cell 293.