Effect of Interleukin-10 on the Phenotype Cultured Human Dendritic Cells by Flow Cytometry of Three Fluorescence Marker

Objective The present study was designed to detect the influence of interleukin (IL)-10 upon the morphologic features of cultured human dendritic cells (DCs), to investigate the method and the meaning of phenotype examined by flow cytometry of three fluorescence marker. Methods Cord blood CD34 + stem cells were isolated and cultured in IMDM medium with SCF、GM-CSF、TGF-β_ 1 、Flt-3、TNF-α. DCs were interfered by recombination of human IL-10. HLA-DR、CD1a、CD11c、CD83、CD80 and CD86 were assayed by flow cytometry of three fluorescence marker(FITC-、PE-、CY-).Results The expression of CD11c、CD83、CD80 and CD86 was lower in IL-10 group. Conclusions IL-10 might inhibit the expression of adhesion and co-stimulation molecules. It was presumed that IL-10 might adjust the antigen presenting function of DCs. In addition, by using the method of three fluorescence marker, we could save DC number needed. Moreover this method is fast and exact, which is deserved to be applied.