The effect of Caspase-8 and Bcl-2 in the apoptosis induced by PIG11 protein in human hepatocellular carcinoma HepG2 cells

Objective:Using the HepG2 cell line low expressing PIG11gene and the HepG2 cell line high expressing PIG11gene which was constructed before by our research group,to research on the function of PIG11 gene in liver cancer cell and the role of which to promote apoptosis.Explore the mechanism of expression PIG11 gene for HepG2 cells lead to apoptosis.Methods:To culture five groups of cell : HepG2 cell,pLXSN-PIG11-HepG2 cell,pLXSN-HepG2 cell,miR-PIG11-HepG2 cell,miR-HepG2 cell,and compare apoptosis of five groups of cell using Hoechst 33342 dyeing fluorescence,flow cytometer scans(FACS),and Western blot analysis.Results:Chromatin condensation,nuclear fragmentation and apoptotic body were observed in pLXSN-PIG11-HepG2 cell group.Flow cytometer scans showed that the apoptosis ratios of HepG2,miR-PIG11-HepG2,miR-HepG2,pLXSN-PIG11-HepG2,pLXSN-HepG2 were 5.72%±0.81%,1.34%±0.71%,5.10%±0.40%,34.83%±2.29%,5.34%±0.60%.The apoptosis ratio of HepG2 cell with PIG11 gene high expression was higher while that of HepG2 cell with PIG11 gene low expression was the opposite than others.The expression of Caspase-8 was higher while that of HepG2 cell with PIG11 gene low expression were lower than others.And the expression of Bcl-2 was lower while that of HepG2 cell with PIG11 gene low expression was higher than others.Conclusion:The apoptosis ratio of HepG2 cell with PIG11 gene high expression was higher.HepG2 cells apoptosis induced by PIG11 was related to Caspase-8 and Bcl-2.