Expression changes of the hypoxia induction factor-1αin Hela cell under hypoxia condition

Objective:To investigate the changes of the expression of hypoxia induction-1a(HIF-1a)of Hela cell under different hypoxia condition via molecular biology and biochemistry technology.Methods:Hela cells were cultured under normal condition;the oxygen content was20%.In the normal control group,Hela cells were cultured under normal condition for96h.The cells of the experiment groups were cultured under different oxygen condition for96h.The oxygen content were10%,5%,3%,1%.The mRNA and protein expression of HIF-1a of Hela cells were detected by reverse transcription-polymerase chain reaction analyses(RT-PCR)and western blot analysis respectively.The activity of SOD and the content of MDA were detected by biochemistry technology.Results:There were some levels of mRNA and protein expression of HIF-1a in Hela cells under normal condition.The mRNA and protein expression of HIF-1a in Hela cells under hypoxia condition increased dramatically(2.471～5.474,P0.01).The mRNA and protein expression of HIF-1a in Hela cells under3%conditions were the highest.In the experiment groups,the activity of SOD decreased dramatically(t=3.337～6.152,P0.01),but the content of MDA increased(t=3.326～5.486,P0.01).Conclusion:The mRNA and protein expression of HIF-1a of Hela cells inreased dramatically under hypoxia condiˉtion.The expression and changes of HIF-1a of Hela cells had close relationship with the increasing of the free radical in the culture media.