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2007•Chinese Journal of HemorheologyRequires access

Study of Adenovirus-Mediated HSV-tk Gene Therapy for Bladder Cancer Cell Line

Zhenhua Guo

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Abstract

Objective To study the new way of adenovirus-mediated gene therapy for bladder cancer.Methods and Results We used the replication-defective adenovirus carring the HSV-tk gene(Ad.tk) under control of the cytomegalovirus(CMV).In vitro experiments,the HTB9 cells transferred by tk gene were killed in the presence of GCV treatment.When tk+ cells were mixed with their parental cells at various ratios,GCV not only killed tk+ cells,but neighboring cells without tk gene also killed by bystander effect.Conclusion Adenovirus-mediated HSV-tk/ GCV system may be used as apromising suicide gene therapy for bladder carcinoma.

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What this paper is about

Objective To study the new way of adenovirus-mediated gene therapy for bladder cancer.Methods and Results We used the replication-defective adenovirus carring the HSV-tk gene(Ad.tk) under control of the cytomegalovirus(CMV).In vitro experiments,the HTB9 cells transferred by tk gene were killed in the presence of GCV treatment.When tk+ cells were mixed with their parental cells at various ratios,GCV not only killed tk+ cells,but neighboring cells without tk gene also killed by bystander effect.Conclusion Adenovirus-mediated HSV-tk/ GCV system may be used as apromising suicide gene therapy for bladder carcinoma.

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Available abstract

Objective To study the new way of adenovirus-mediated gene therapy for bladder cancer.Methods and Results We used the replication-defective adenovirus carring the HSV-tk gene(Ad.tk) under control of the cytomegalovirus(CMV).In vitro experiments,the HTB9 cells transferred by tk gene were killed in the presence of GCV treatment.When tk+ cells were mixed with their parental cells at various ratios,GCV not only killed tk+ cells,but neighboring cells without tk gene also killed by bystander effect.Conclusion Adenovirus-mediated HSV-tk/ GCV system may be used as apromising suicide gene therapy for bladder carcinoma.

Key concepts: Genetic enhancement, Bystander effect, Suicide gene, Virology, Cancer research, Gene, Cell culture, Viral vector

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