Effect of defibrase on focal cerebral ischemia in rats

AIM: To observe the therapeutic effect of defibrase after focal cerebral ischemia in male Wister rats. METHODS:Intraluminal suture method was used to develop reversible middle cerebral artery occlusion (MACO). Sixty focal cerebral ischemia rats were randomly divided into ten groups(one group six rats). Five groups were injected intravenously Hai wang defibrase 8 U·kg 1 at 0.5, 3,6,9,12 h after cerebral ischemia. The other five groups (control groups) were injected intravenously equivalent saline at the same time. Clinical neurological deficits scale were completed at 24 h after MCAO . Then all rats were killed. Cerebral infarction was examined microscopically in HE stained sections. Infarction volume was measured by using 2,3,5 triphenyltetrazolium chloride (TTC) staining. RESULTS:The rats were injected intravenously defibrase in 0.5, 3 h after MCAO. Their clinical neurological deficits scale was 0.8 ± s 0.5, 1.3±0.5, and 3.0±0.9, 3.2±1.0, respectively. The infarction volume was (119±39) mm 3, (141±62)mm 3, and (291±56)mm 3, (297±39)mm 3, respectively. Which were all significantly reduced in comparison with the control groups( P 0.01).Pathologic change was also significantly improved. There were no statistically significant differences at 6, 9,12 h after MCAO. Both treating groups and the control groups were found punctual hemorrhage under the microscop, but cerebral hemorrhage wasn't increased in defibrase treatment group. CONCLUSION: Defibrase can reduce the infarction volumn without the increase of hemorrhage at cerebral ischemia early stage.