Self-assembling protein microarray— a new tool for functional proteomics

Traditional protein microarrays require time-consuming processes of protein production and purification since the protein activities are not stable and the array products could not be stored properly for a long time.Self-assembling protein microarrays utilized cell-free expression system and DNA immobilization technique,which allowed multiple-protein expression simultaneously and in situ immobilization on the surface of arrays.This technology effectively overcomes the problems of traditional protein microarrays,and provides a useful tool for functional proteomics analysis.Currently,self-assembling protein microarrays are primarily used for screening protein-protein interactions,identifying immunodominant antigens and so on.This review focused on the recent advancement and application research on self-assembling protein microarrays.