The effects of P38MAPK signaling pathway inhibitor SB203580 on hypoxia-reperfusion of liver cells

Objective To investigate the effects of P38MAPK inhibitor SB203580 on hypoxia-reperfusion process of human hepatoma cell line HepG2cells and normal human cell lines LO2cells. Methods Experiment were divided into normal control group,hypoxia control group,SB203580 + normal group and SB203580 + hypoxia group,hypoxia Cultured cells 24h and reoxygen 1h,applied Western Blot,MTT,scratch test,Transwell experiments and AnnexinV- FITC / PI staining to test the expression of P38 protein,cell proliferation,migration,invasion and apoptosis respectively. Results Compared with normal control group,the HepG2cells apoptosis rate and P38MAPK phosphorylation levels of the hypoxia group were increased,while compared with hypoxia control group,the HepG2cells apoptosis rate was increased and P38MAPK phosphorylation levels was decreased of the SB203580 + hypoxia group; After 48h in scratch test,the control group of HepG2cells moved to the center of the scratch,and SB203580 + hypoxia group was inhibited; After 24h in migration assay,SB203580 + hypoxia group of HepG2cells' invasive ability was inhibited compared with the control group( P 0. 05); In Annexin V-FITC / PI assay,the cell apoptosis rates of the SB203580 + hypoxia group showed a significant increase in HepG2cells and decrease in LO2cells when compared with control group( P 0. 01); In MTT assay,SB203580 affected the HepG2cells and LO2cells' Proliferation inhibition rate,and depended on the time and concentration. Conclusion SB203580 through blocking P38MAPK signal transduction pathway specifically,have a protective effect on LO2cells and promote the apoptosis,inhibit the proliferation and migration,reduce invasion capabilities on HepG2cells.