The role of miR-200c in the apoptosis of gastric cancer cell induced by CIK cells

Objective To observe the effect of cytokine-induced killer cells(CIK cells) on the apoptosis of gastric cancer(GC) cells,and explore the role of microRNA-200c(miR-200c) in this process.Methods MiR-200c was determined by real time quantitative PCR,and the apoptosis was detected by flow cytometry in GC cells(MKN-45 and BGC-803).psiCHECK2-FAP-1 3’-UTR containing the binding site of miR-200c was constructed,and then transfected into GC cells.Luciferase activity of FAP-1(Fas-associated phosphatase-1) was determined with the assistance of dual luciferase report system.Results CIK cells promoted GC cell apoptosis(P 0.01) and the expression of miR-200c [(2.10 ±0.25) fold,P 0.05],and miR-200c inhibitor might block this process(P 0.05).Moreover,miR-200c mimics induced GC cell apoptosis(P 0.05).The similar trend was also observed in BGC-803 cells.MiR-200c targeted at the site of FAP-1 3’-UTR,and CIK cells decreased the luciferase activity of GC cells [MKN-45:(49.67 ± 2.36) %,P 0.01;BGC-803:(43.86 ± 4.41) %,P 0.01].Conclusion CIK cells could enhance GC cell apoptosis by upregulating miR-200c,which may provide a new database to elucidate GC therapy using CIK cells.