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2010•Chinese Journal of Health Laboratory TechnologyRequires access

Study on detection of Salmonella with Helicase-dependent isothermal DNA amplification technology

Yunguo Liu

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Abstract

Objective:To establish a new rapid method to detect Salmonella of sample which based on Helicase-dependent isothermal DNA amplification(HDA).Methods: A highly specific set of primers were synthesized to target the invA gene of Salmonella.Specific and sensitivity was tested which was compared with PCR method.Results: Helicase-dependent isothermal DNA amplification method that can rapidly detect Salmonella has been established.The sensitivity was 460 pg/tube which was similar to the result of PCR method.Conclusion: Detecting Salmonella with HDA is a rapid,user-friendly,specific,sensitive method.It′s very suitable for primary laboratory use,with a broad prospect.

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What this paper is about

Objective:To establish a new rapid method to detect Salmonella of sample which based on Helicase-dependent isothermal DNA amplification(HDA).Methods: A highly specific set of primers were synthesized to target the invA gene of Salmonella.Specific and sensitivity was tested which was compared with PCR method.Results: Helicase-dependent isothermal DNA amplification method that can rapidly detect Salmonella has been established.The sensitivity was 460 pg/tube which was similar to the result of PCR method.Conclusion: Detecting Salmonella with HDA is a rapid,user-friendly,specific,sensitive method.It′s very suitable for primary laboratory use,with a broad prospect.

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Available abstract

Objective:To establish a new rapid method to detect Salmonella of sample which based on Helicase-dependent isothermal DNA amplification(HDA).Methods: A highly specific set of primers were synthesized to target the invA gene of Salmonella.Specific and sensitivity was tested which was compared with PCR method.Results: Helicase-dependent isothermal DNA amplification method that can rapidly detect Salmonella has been established.The sensitivity was 460 pg/tube which was similar to the result of PCR method.Conclusion: Detecting Salmonella with HDA is a rapid,user-friendly,specific,sensitive method.It′s very suitable for primary laboratory use,with a broad prospect.

Key concepts: Salmonella, Loop-mediated isothermal amplification, Helicase, DNA, Molecular biology, Recombinase Polymerase Amplification, Sensitivity (control systems), Biology

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