2006•Tianran chanwu yanjiu yu kaifaRequires access
Identification and Activity of Antibacterial Substance from Xenorhabdus nematophila var.Pekingense
Huang Wu-ren, Yang Xiufen, Yang Huaiwen
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Abstract
Xenorhabdus nematophilus var.pekingensis CB6 strain is symbiotic bacteria with Steinernema carpocapsaea and is carried in the intestine of the infective juvenile(IJ) stage of the emtomopathogenic nematode.It is significant to identify and bioassay antibacterial substance from metabolites of Xenorhabdus nematophila var.pekingense.The antibacterial substance was identified as Xenocoumacin 1 by()~1H NMR,()~(13)C NMR,mass spectrum,infrared and ultraviolet absorption spectrum.The substance inhibited broad-spectrum plant pathogenic fungi.The mycelial growth of Phytophthora boehmeriae,P.melonis,P.infestance,P.capsici,P.cactorum Botrytis cinerea and Alterinaria alternata was completely inhibited at 10 μg/mL concentration.The EC_(50) of 6 fungi species was 0.25～4.17 μg/mL.The inhibitory activity to Phytophthora spp.was the highest among tested 12 fungi species.The antibacterial substance could result in abnormal branching increasement,hyphae tip inflation and cytoplasm leak of P.infestance.The research will establish a base for development of novel biofungicide.