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The change of p38MAPK in the apoptosis of K562 cells induced by topotecan

Yuehua Zhao

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Abstract

Objective To study the signal transduction in apoptosis of K562 cells induced by topotecan.Methods After K562 cells were treated by topotecan in 24 hours,the apoptosis effect was evaluated by FCM and DNA fragment.p38MAPK content was detected by Western blot and p38MAPK activity was assayed by the measurement of the incorporation of 32P from [γ-32P] ATP into peptide substrates.Results An apoptosis peak was found in the K562 cell cycle by FCM and the apoptosis rate was 16.9%.The content of DNA fragment was(45.9±1.0)%.p38MAPK content was 4.9 times and p38MAPK activity was 4.1 times higher than that in the control group,respectively(P 0.05).Conclusion Topotecan could induce apoptosis of K562 cells by activating p38MAPK.

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Objective To study the signal transduction in apoptosis of K562 cells induced by topotecan.Methods After K562 cells were treated by topotecan in 24 hours,the apoptosis effect was evaluated by FCM and DNA fragment.p38MAPK content was detected by Western blot and p38MAPK activity was assayed by the measurement of the incorporation of 32P from [γ-32P] ATP into peptide substrates.Results An apoptosis peak was found in the K562 cell cycle by FCM and the apoptosis rate was 16.9%.The content of DNA fragment was(45.9±1.0)%.p38MAPK content was 4.9 times and p38MAPK activity was 4.1 times higher than that in the control group,respectively(P 0.05).Conclusion Topotecan could induce apoptosis of K562 cells by activating p38MAPK.

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Available abstract

Objective To study the signal transduction in apoptosis of K562 cells induced by topotecan.Methods After K562 cells were treated by topotecan in 24 hours,the apoptosis effect was evaluated by FCM and DNA fragment.p38MAPK content was detected by Western blot and p38MAPK activity was assayed by the measurement of the incorporation of 32P from [γ-32P] ATP into peptide substrates.Results An apoptosis peak was found in the K562 cell cycle by FCM and the apoptosis rate was 16.9%.The content of DNA fragment was(45.9±1.0)%.p38MAPK content was 4.9 times and p38MAPK activity was 4.1 times higher than that in the control group,respectively(P 0.05).Conclusion Topotecan could induce apoptosis of K562 cells by activating p38MAPK.

Key concepts: Topotecan, Apoptosis, K562 cells, Western blot, Molecular biology, Chemistry, DNA, Biology

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