Preparation of Functional Monoclonal Antibody Against Human IL-11 and Analysis of Its Biological Characteristics

RhIL-11 was used as immunogen to immunize BALB/c mouse Two hybridoma cell lines,which secrete specific McAb against human IL-11,were obtained by using cell fusion,limited coloning assay,and ELISA screening The specificity of McAb was verified by Western blotting and the subsets of IgG,which the McAb belong to,were indicated by quickly test-paper analysis The biological function of the McAb in the proliferation of B9-11 cells was measured by MTT assay in vitro Two anti-human IL-11 McAb,5A2 and 1G12,which is belonging to IgG1 and IgG2 subset respectively,were obtained Both of them can inhibit the proliferation of an IL-11-dependent cell line,B9-11 cell line respectively,when IL-11 was present in culture system Thus two neutralizing anti-human IL-11 specific McAb were successfully obtained -