Monoclonal antibodies directed to human brain acetylcholinesterase

BALB/c mice were immunizedsubcutaneously, intramuscularly and intradperitoneallyfor five times with the purified human cerebrumacetylcholinestenise(AChE).Splenocytes of the immunlzedmice were fused with SP2/O-Ag14 mouse myeloma cells.Three hybridomas(B10,N11,E11)have been developed,the dilution titers of the ascites antibodies were:96000,1:48 000 and 1:24000 respectively. The subclasses of themonoclonal antibodies and the chromosomes of hybridomacells were also determined.All the three monoclonalantibodies remarkably reacted with the native TorpedoAChE, however no obvious cross immunoreactivity couldbe detected with the humam red blood cell membraneAChE. The hybridoma cell lines were stored in liquid nitro-gen after three to six months of propagation for stability.The cells grew well and fairly reserved the antibody-se-creting capacity at resuscitation one year later.