Experimental study on apoptosis of cultured human pancreatic carcinoma cells Bxpc-3 induced by paclitaxel in vitro

Objective To explore the effect of paclitaxel(PA)on apoptosis of cultured human pancreatic carcinoma cells Bxpc-3 in vitro. Methods Bxpc-3 cells were cultured separately with RPMI1640 culture medium(control group)and with 100,50,25 mg/ml of PA(PA groups).Bxpc-3 cell proliferation was detected with the modified MTT method.The Bxpc-3 cell DNA ploidy distribution and apoptotic rate were measured by flow cytometry. Results Compare with the controls,the Bxpc-3 cell proliferation was significantly inhibited in a dose dependent manner after treated with PA(100,50,25 mg/ml)for 24 h;the percentage of Bxpc-3 cell population in the G0/G1 phase increased after treated with 100,50 mg/ml PA,but reduced in S phase,and proliferation index(PI)also reduced.Cell apoptosis index(AI)was higher in 100,50 mg/ml PA 48 h group than in control group.AI/PI in 100,50 mg/ml PA 48 h group were 6 and 2.5 times as much as that in control group. Conclusion PA can inhibit cell proliferation and induce cell apoptosis of human pancreatic carcinoma cells Bxpc-3.