2011Northern HorticultureRequires access

Study on Properties and Inhibition Methods of Polyphenol Oxidase from Yacon

Wang Ling-ling

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Abstract

Polyphenol oxidase(PPO) was extracted from yacon roots,and its properties were studied.Its optimum activity pH was 6.4.The optimum temperature was 30℃.The PPO was inactivated when incubated at 80,90,100℃for 300,240,200 s respectively.NaCl and sucrose had no effect on PPO activity.The activity was inhibited by ascorbic acid, citric acid,lemon juice and orange juice.Lemon juice and orange juice could be applied in yacon processing as safe, harmless and natural inhibition methods of enzymatic browning.vitamin C and citric acid had highly significant effects on PPO activity.orange juice,the interaction of vitamin C and citric acid had significant effects.

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What this paper is about

Polyphenol oxidase(PPO) was extracted from yacon roots,and its properties were studied.Its optimum activity pH was 6.4.The optimum temperature was 30℃.The PPO was inactivated when incubated at 80,90,100℃for 300,240,200 s respectively.NaCl and sucrose had no effect on PPO activity.The activity was inhibited by ascorbic acid, citric acid,lemon juice and orange juice.Lemon juice and orange juice could be applied in yacon processing as safe, harmless and natural inhibition methods of enzymatic browning.vitamin C and citric acid had highly significant effects on PPO activity.orange juice,the interaction of vitamin C and citric acid had significant effects.

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Available abstract

Polyphenol oxidase(PPO) was extracted from yacon roots,and its properties were studied.Its optimum activity pH was 6.4.The optimum temperature was 30℃.The PPO was inactivated when incubated at 80,90,100℃for 300,240,200 s respectively.NaCl and sucrose had no effect on PPO activity.The activity was inhibited by ascorbic acid, citric acid,lemon juice and orange juice.Lemon juice and orange juice could be applied in yacon processing as safe, harmless and natural inhibition methods of enzymatic browning.vitamin C and citric acid had highly significant effects on PPO activity.orange juice,the interaction of vitamin C and citric acid had significant effects.

Key concepts: Yacón, Citric acid, Polyphenol oxidase, Browning, Chemistry, Food science, Sucrose, Orange (colour)

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