Cloning and Prokaryotic Expression of IL-2 Gene of Luoyang Local Chicken in E.coli

A cDNA encoding Chicken interleukin-2(IL-2)was amplified by reverse transcription-polymerase chain reaction(RT-PCR)from Luoyang local Chicken embryo spleenocytes . Sequences analysis showed that the target gene was Chicken IL-2 and no termination codon.The IL-2 gene was inserted into the bacterial plasmid PET-28a, resulting in the construction of PET-28a-IL2 prokaryotic expression plasmids. The recombiant plasmid PET-28a-IL2 containing IL-2 gene were identified by restriction enzymes analysis and PCR method and sequenced to confirm its rightness. The recombinant fusion protein was highly expression in E. coli BL21(DE_(3))four hours later induced by IPTG and the molecular weight is about 14 ku. These provide a basis for the study on the biological property of chicken IL-2.