2005•Journal of Science of Teachers' College and UniversityRequires access

Factors influencing Agrobacterium tumefaciens-mediated transformation in Nodulisporium sylviforme

WU Chen-wei

Abstract

Agrobacterium tumefaciens-mediated transformation system was used to study on factors influencing transformation in nodulisporium sylviforme HQD_(33).The results showed that the transformation efficiency was correlated with A.tumefaciens strains,the growing condition of A.tumefaciens strains,concentration of conidia,co-cultivation time and acetosyringone induction.Through setting up Agrobacterium tumefaciens-mediated transformation system,the production of taxol will be increased greatly.These findings should facilitate future study of nodulisporium sylviforme HQD_(33).

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Key concepts: Acetosyringone, Agrobacterium tumefaciens, Transformation (genetics), Agrobacterium, Biology, Microbiology, Genetics, Gene