2012•Zhongguo linchuang yaolixue yu zhiliaoxueRequires access
Effects of curcumin pretreatment on heme oxygenase-1 in lung injury induced by ischemia-reperfusion of hind limbs in rats
Yang Jun
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Abstract
AIM:To investigate the effects of curcumin pretreatment on heme oxygenase-1(HO-1) in lung injury induced by ischemia-reperfusion(I/R) of hind limbs in rats.METHODS: The model of lung injury was established by occlusion of bilateral femoral arteries for 2 h followed by 3 h reperfusion in rat.Sixty SD rats were randomly divided into five equal groups: Sham group,Curcumin group(Cur group),I/R group,Curcumin+I/R group(Cur-I/R group),Zinc protoporphyrin IX+Curcumin+I/R group(ZnPP-Cur-I/R group).ZnPP-Cur-I/R group received ZnPP 20 mg/kg via intraperitoneal injection 24 h before ischemia.Cur,Cur-I/R and ZnPP-Cur-I/R groups received curcumin 200 mg/kg respectively via intraperitoneal injection 2 h before ischemia.I/R of hind limbs was induced in three I/R groups.The lungs were removed for pathologic examination and determination of the lung Wet/dry weight ratio(W/D),lung injury score and polymorphonuclear neutrophil(PMN) count.The expression of HO-1 mRNA and protein in lung tissues was detected using RT-PCR and Western blot analysis respectively.RESULTS:Alveolar edema,localized pulmonary atelectasis and large amount of PMN infiltration were found in I/R group and were ameliorated in Cur-I/R group.Lung injury score,W/D ratio and PMN count were increased in I/R group as compared with Sham group(P0.01).Lung injury score,W/D ratio and PMN count were significantly decreased in Cur-I/R group as compared with I/R group(P0.01).HO-1 mRNA and protein expression was significantly up-regulated in Cur-I/R group than in Sham and I/R groups(P0.01).Compared with Cur-I/R group,the beneficial effects of curcumin were abolished,lung injury score,W/D ratio and PMN count were significantly increased,and expression of HO-1 mRNA and protein was down-regulated in ZnPP-Cur-I/R group(P0.01).CONCLUSION: Curcumin pretreatment has protective effects against lung injury induced by I/R of hind limbs in rats through up-regulation of HO-1 expression in the lung tissues.