Effects of endothelial lipase on ICAM-1 expression in human umbilical vein endothelial cells

Objective Endothelial lipase (EL) modulates the monocyte adhesion to the endothelial cells. We explore the effect of EL on the expression of adhesion molecules in endothelial cells. Methods Human umbilical vein endothelial cells (HUVECs) were treated with TNF-α (10 ng/ml), TNF-α (10 ng/ml)+anti-EL antibody (0, 2.5, 5, 10, 25, 50 ng/ml, respectively) or serum-free media, then the expression levels of intercellular adhesion molecule-1 (ICAM-1) mRNA were detected by RT-PCR. Results After treated with TNF-α, the ICAM-1 mRNA levels expressed by HUVECs were significantly increased. These effects were significantly antagonized by anti-EL antibody in a dose-dependent manner. Conclusion EL expressed in HUVECs stimulated by TNF-α can promote the expressions of adhesion molecules in endothelial cells, indicating EL may participate in the pathophysiologic progress of coronary artery disease.