Cultivation and observation of basilar membrane of cochlea from neonatal mouse in vitro

Objective To establish the cultivation model in vitro of basilar membrane of cochlea in neonatal Kunming mouse and observe the histological structure of basilar membrane of cochlea.Methods The basilar membrane of cochleae from Kunming mouse of postnatal 2-3 days was taken and positioned in 24-well plate.The specimens were cultured in dulbecco's modified eagle's medium(DMEM) /F12 medium which containing volume ratio 10% bovine serum albumin.Tetramethylrhodamine isothiocyanate-labeled phalloidine staining was used to stain hair cell,while neurofilmnent protion was used to stain auditory nerve and spiral ganglion neurons.The growth of cochlear hair cells,auditory nerve and spiral ganglion neurons were observed.Results The cochlear basilar membrane has been cultured for six days.The structure of basilar membrane was intact.The growths of cochlear hair cells and spiral ganglion neurons were good.Conclusion This culture method is simple and effective,which can be applied to the experiment of cochlear basilar membrane in vitro culture.