Effect of activated JNK on pulmonary fibroblasts secretion matrix induced by TGF-β_1 in vitro

Objective: To investigate the effect of activated c-Jun NH2-terminal kinase(JNK)、P38 on pulmonary fibroblasts secretion matrix induced by transforming growth factor β1(TGF-β1).Methods: The human pulmonary fibroblasts cultured on plastic plates were divided into following groups: TGF-β1,TGF-β1+ SB203580,TGF-β1+DMSO,TGF-β1+ SB600125,Control.The expression of total JNK(T-JNK),phosphorylated JNK(p*-JNK),total P38(T-P38) and phosphorylated P38(p*P38) were assessed by Western blot,FN and LN were detected by Western blot. Results: A marked increase in p*-JNK was seen in pulmonary fibroblasts while the expression of p*-P38 had no obvious change after being induced by TGF-β1.Protein level of FN and LN was increased markedly at 48 hours after the treatment of TGF-β1.Addition of JNK inhibitor SB600125 largely abrogated the effect of TGF-β1. Conclusion: TGF-β1 can induce the secretion matrix of pulmonary fibroblasts via JNK signal pathway.