[Study on the gene expression patterns of KANGLAITE in anti-lung metastasis of LA795 mouse].

BACKGROUND: To screen the differential expression genes of KANLAITE in anti-tumor metastasis. METHODS: mRNA was extracted and purified from the lung of the mouse with LA795 lung metastasis, hybridized respectively on 4 096-gene chip. cDNA microarray was scanned for the fluorescent signals and analyzing difference expression. RESULTS: Twenty-seven differential expressed genes were obtained. Among these genes, 25 were up-regulated and 2 were down-regulated. Twelve of them were Mus musculus cDNA clone. Six genes related with genesis, development and metastasis of tumor. CONCLUSIONS: cDNA microarray for analysis of gene expression patterns is a powerful method to identify differential expressed genes. In this study, 6 genes are thought to be associated genes of KANGLAITE in anti-tumor metastasis.