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2011•Journal of China Pediatric Blood and CancerRequires access

Mechanism research on apoptosis of K562 cells induced by 2-methoxyestradiol

Ruijuan Li

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Abstract

Objective To investigate mechanism of apoptosis of K562 cells induced by 2-methoxyestradiol(2-ME).Methods K562 cells were treated with 2-ME(0,5,10,20,40 μmol/L) for 48 h.The cell apoptosis was measured by flow cytometry.Expressions of Bcl-2,Bax and Caspase-3 proteins in K562 cells were detected by western blot.Results(1) 2-ME with various concentrations(0,5,10,20,40 μmol/L) could induce the cell apoptosis of K562 cells,and the apoptosis rate of K562 cells significantly increased(P0.05) in a dose-dependent manner.(2) Bcl-2 protein expression decreased significantly in 2-ME treated group compared with the control group(P0.05),while Bax and Caspase-3 protein expressions were elevated significantly in 2-ME treated group compared with the control group(P0.05).Conclusion The apoptosis of K562 cells induced by 2-methoxyestradiol might associated with the mitochondrial pathway.

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Objective To investigate mechanism of apoptosis of K562 cells induced by 2-methoxyestradiol(2-ME).Methods K562 cells were treated with 2-ME(0,5,10,20,40 μmol/L) for 48 h.The cell apoptosis was measured by flow cytometry.Expressions of Bcl-2,Bax and Caspase-3 proteins in K562 cells were detected by western blot.Results(1) 2-ME with various concentrations(0,5,10,20,40 μmol/L) could induce the cell apoptosis of K562 cells,and the apoptosis rate of K562 cells significantly increased(P0.05) in a dose-dependent manner.(2) Bcl-2 protein expression decreased significantly in 2-ME treated group compared with the control group(P0.05),while Bax and Caspase-3 protein expressions were elevated significantly in 2-ME treated group compared with the control group(P0.05).Conclusion The apoptosis of K562 cells induced by 2-methoxyestradiol might associated with the mitochondrial pathway.

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Available abstract

Objective To investigate mechanism of apoptosis of K562 cells induced by 2-methoxyestradiol(2-ME).Methods K562 cells were treated with 2-ME(0,5,10,20,40 μmol/L) for 48 h.The cell apoptosis was measured by flow cytometry.Expressions of Bcl-2,Bax and Caspase-3 proteins in K562 cells were detected by western blot.Results(1) 2-ME with various concentrations(0,5,10,20,40 μmol/L) could induce the cell apoptosis of K562 cells,and the apoptosis rate of K562 cells significantly increased(P0.05) in a dose-dependent manner.(2) Bcl-2 protein expression decreased significantly in 2-ME treated group compared with the control group(P0.05),while Bax and Caspase-3 protein expressions were elevated significantly in 2-ME treated group compared with the control group(P0.05).Conclusion The apoptosis of K562 cells induced by 2-methoxyestradiol might associated with the mitochondrial pathway.

Key concepts: Apoptosis, K562 cells, 2-Methoxyestradiol, Western blot, Flow cytometry, Chemistry, Molecular biology, Cancer research

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