Study on the inhibition of proliferation on gastric cancer cells by RNA interference targeting to IGF-IR

Objective To observe the expression of IGF-ⅠR on human gastric cancer MGC803 cells inhibited by special siRNAs,and investigate the following effects of the cell proliferation.Methods Two different siRNAs targeted to IGF-ⅠR were designed,synthesized and transfected into MGC803 cells,the expression of IGF-ⅠR were detected by Western Blot before and after the transfection,then the cell proliferation were examined by MTT,the growth curve was obtained.Results Intensive expression of IGF-ⅠR was shown in MGC803 cells.The inhibition ratio of IGF-ⅠR protein of interfering groups(siRNA2-low dose group,siRNA2-high dose group,siRNA1 group) were 64.41%±4.11%,74.14%±6.15%,89.8%±4.10% respectively 48 hours after transfection;The cell number gradually decreased by 49.9%±1.2%,45.9%±4.4%,39.1%±5.1%,29%±4.0% at the same time.Conclusion Special siRNAs can remarkably inhibit the expression of IGF-ⅠR in gastric cancer cells,the cell proliferation was decreased greatly.