Intervention Effect of Cathepsin B Inhibitor on Expression of Clusterin in Neonatal Rats Hippocampus after Recurrent Neonatal Seizures

Objective To explore the expression of Clusterin(CLU) in neonatal rats hippocampus after recurrent neonatal seizures and the intervention efficacy of Cathepsin B inhibitor(CBI). Methods Six-day-old Sprague-Dawley(SD) rats were randomly divided into 3 groups,including recurrent seizure group(RS group,n=6),CBI-treated seizure group(CBI group,n=6),and control group(n=6).Rats in RS group were subjected to 5 attacks of seizures induced by using flurothyl for 9 d,at intervals of 30 min,while rats in control group did without flurothyl.In CBI group,CBI(2 μL,0.5 g·L-1)was administered every day before seizures were induced.Western blot was employed to determine Clusterin expression at P35.SPSS 17.0 software was used to analyze the data. Results There was higher expressions of Clusterin in RS group(1.19±0.10) than that in control group(0.64±0.24)(P0.01).There was lower expression of Clusterin in CBI group(0.80±0.16) than that in RS group(P0.01).There was no significant difference between CBI group and control group. Conclusions Increased Clusterin expression may be involved in molecular mechanism induced by brain injury after recurrent seizures.CBI has significant intervention efficacy on the expression of Clusterin.