CD_(40) ligandization inhibits in vitro proliferation of breast cancer cell line (M231) and promotes its sensetivity to adriamycin

OBJECTIVE:To investigate the effect of CD_ 40 ligandization in proliferation and apoptosis of breast cancer cell line M231 in vitro. METHODS: The expression of CD_ 40 and its ligand on breast cancer cell line(M231) were determined indirect immunofluorescence assay with flow cytometry (FMC) analysis after M231 cells were incubation with interferon-γ (IFN-γ) and basic fibroblast growth factor (bFGF) for 18 hours. Then M231 cells cultured with CD_ 40 agonsitic monoclone antibody, adriamycin alone or in combinations for 72 hours and proliferation of M231cells was determined by MTT assay. FCM was employed to study the cells' death or apopotosis with PI-Annexin V assay. RESULTS: M231 cells expressed CD_ 40 (57.3±4.6) %, No CD_ 40 ligand are expressed. The expression of CD_ 40 on M231 can be up-regulated by IFN-γ (88.5±5.6)%, there are statistics difference,P=0.023. The proliferation of M231 may be inhibited after incubated with CD_ 40 agonsitic monoclone antibody,P=0.014. Combined CD_ 40 agonsitic monoclone antibody with adriamycin may obviously inhibit proliferation of to M231,P=0.006 7. CD_ 40 ligandization may promote the death and apoptosis of M231. CONCLUSION: IFN-γ and CD_ 40 ligandization can enhance the effect of adriamycin to M231.