Steam explosion pretreatment strategies for enhancing enzymatic hydrolysis of reed

Reed is a kind of high biomass plants.It can be used as important raw material for bio-ethanol production.In this research,several pretreatments(steam explode,alkali-catalyzes steam explosion,acid-catalyzes steam explosion) and enzymatic saccharification procedures were studied.Hydrolystates from three pretreated(dilute acid,sodium hydroxide and acid-catalyzes steam explosion) reed were analyzed.The results showed that acid-catalyzes steam explosion was the best pretreatment in terms of resulting reducing sugar concentration in the hydrolysate after enzymatic hydrolysis.The conditions of acid-catalyzes steam explosion was pretreated by soak in 1.0% dilute acid,then performed steam explosion in a retention time of 120s and a steam-pressure of 2.0MPa.The highest rate of hydrolysis and enzymatic saccharification were 37.8% and 82%,respectively.The analysis of hydrolysate for pretreatment and enzymatic saccharification from reed indicated that acid-catalyzes steam explosion pretreatment could produced more fermentable sugar,and the contents of the glucose and xylose in enzymatic hydrolysate were 52.75% and 43.39%,respectively.