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2006•Journal of Biological ControlRequires access

Optimization of Bacillus subtilis B908 Fermentation Medium

Mei Ru-hong

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Abstract

Plackett-Burman design and response surface analysis were used to optimize the fermentation medium of Bacillus subtilis B908.Based on the results of Plackett-Burman design,important factors affecting the yield of Bacillus subtilis B908 were corn flour,bean flour and CaCO_3.Then,the three factors were further optimized using response surface analysis.The optimized fermentation medium allowed the production to be increased from 6.6×10~9spores ml~(-1) to 1.18×10~(10)spores ml~(-1).

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What this paper is about

Plackett-Burman design and response surface analysis were used to optimize the fermentation medium of Bacillus subtilis B908.Based on the results of Plackett-Burman design,important factors affecting the yield of Bacillus subtilis B908 were corn flour,bean flour and CaCO_3.Then,the three factors were further optimized using response surface analysis.The optimized fermentation medium allowed the production to be increased from 6.6×10~9spores ml~(-1) to 1.18×10~(10)spores ml~(-1).

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Available abstract

Plackett-Burman design and response surface analysis were used to optimize the fermentation medium of Bacillus subtilis B908.Based on the results of Plackett-Burman design,important factors affecting the yield of Bacillus subtilis B908 were corn flour,bean flour and CaCO_3.Then,the three factors were further optimized using response surface analysis.The optimized fermentation medium allowed the production to be increased from 6.6×10~9spores ml~(-1) to 1.18×10~(10)spores ml~(-1).

Key concepts: Bacillus subtilis, Plackett–Burman design, Fermentation, Food science, Spore, Response surface methodology, Biology, Corn flour

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