2003•Di-Si Junyi Daxue xuebaoRequires access

Construction and preliminary selection and identification of phage display library of anti-human ovarian cancer single chain Fv antibody

Xin Xiao

Abstract

AIM: To construct the phage display library of anti human ovarian cancer single chain Fv antibody. METHODS: The ScFv gene library was constructed by phage display technology. The affinity to antigen was detected by ELISA after panning. RESULTS: Twenty six antigen binding clones were identified from 94 individual phagemid clones. CONCLUSION: In the production of engineering antibodies against human cancer, this strategy may provide an alternative approach.

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Key concepts: Panning (audio), Phage display, Phagemid, Antibody, Antigen, Biology, Peptide library, Selection (genetic algorithm)