ScholarLens Back to search
2008•Unpublished venueRequires access

Detecting DNA Damage of Human Lymphocytes induced by Methotrexate with Comet Assay

Yongxin Lin

Open publisher page 0 citations

Abstract

Objective To assess the DNA damage of human lymphocytes exposed to me thotrexate(MTX) at different doses.Methods Lymphocytes were separated from peripheral blood taken from two healthy donors(one male and another female).Then the lymphocytes were incubated for 6 and 24 hours with 0(solvent control group),5,10,25,50,100μg/ml MTX respectively.The Comet assay was used to detect the tail length(TL) which was served as a indicator of DNA damage as well as tail moment(TM).Meanwhile the dose-effects relationship was analyzed.Results The rate of DNA damage of MTX-exposed group was significantly higher than that of control group.In addition,a dose-effects relationship was found.Conclusion Comet assay is a rapid and simple test to assess DNA damage.The result of this study indicates that MTX can induce DNA damage of human lymphocytes in vitro.

About this research paper

What this paper is about

Objective To assess the DNA damage of human lymphocytes exposed to me thotrexate(MTX) at different doses.Methods Lymphocytes were separated from peripheral blood taken from two healthy donors(one male and another female).Then the lymphocytes were incubated for 6 and 24 hours with 0(solvent control group),5,10,25,50,100μg/ml MTX respectively.The Comet assay was used to detect the tail length(TL) which was served as a indicator of DNA damage as well as tail moment(TM).Meanwhile the dose-effects relationship was analyzed.Results The rate of DNA damage of MTX-exposed group was significantly higher than that of control group.In addition,a dose-effects relationship was found.Conclusion Comet assay is a rapid and simple test to assess DNA damage.The result of this study indicates that MTX can induce DNA damage of human lymphocytes in vitro.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective To assess the DNA damage of human lymphocytes exposed to me thotrexate(MTX) at different doses.Methods Lymphocytes were separated from peripheral blood taken from two healthy donors(one male and another female).Then the lymphocytes were incubated for 6 and 24 hours with 0(solvent control group),5,10,25,50,100μg/ml MTX respectively.The Comet assay was used to detect the tail length(TL) which was served as a indicator of DNA damage as well as tail moment(TM).Meanwhile the dose-effects relationship was analyzed.Results The rate of DNA damage of MTX-exposed group was significantly higher than that of control group.In addition,a dose-effects relationship was found.Conclusion Comet assay is a rapid and simple test to assess DNA damage.The result of this study indicates that MTX can induce DNA damage of human lymphocytes in vitro.

Key concepts: Comet assay, DNA damage, Molecular biology, DNA, Methotrexate, In vitro, Peripheral blood, Lymphocyte

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Detecting DNA Damage of Human Lymphocytes induced by Methotrexate with Comet Assay — Research Paper | ScholarLens