Tissue culture of albus guripingensis
Chen Ai
Abstract
Chen Ai
Abstract
The optimum explants to be gotten were budllets using konjak as inoculating materials.At different dose of hormone,MS+6-BA0.5mg/L+NAA1.0mg/Lappeared to be optimum for callus induction,with an induction rate of 94.9%.For regeneration of plantlets from the callus,and the optimum medium was MS+6-BA1.5mg/L+NAA0.1mg/L,with a differentiation rate of 95.2%.All the regenerated plantlets rooted readily on MS+NAA0.3~0.5mg/L and grew into complete plants.
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The optimum explants to be gotten were budllets using konjak as inoculating materials.At different dose of hormone,MS+6-BA0.5mg/L+NAA1.0mg/Lappeared to be optimum for callus induction,with an induction rate of 94.9%.For regeneration of plantlets from the callus,and the optimum medium was MS+6-BA1.5mg/L+NAA0.1mg/L,with a differentiation rate of 95.2%.All the regenerated plantlets rooted readily on MS+NAA0.3~0.5mg/L and grew into complete plants.
Key concepts: Callus, Explant culture, Inoculation, Tissue culture, Botany, Regeneration (biology), Horticulture, Biology