Protective effect of dihydrolycorine on H_2O_2-induced oxidative stress damage in PC12 cells

Objective: To investigate the protective effect of dihydrolycorine(DL) on H2O2-induced oxidative stress damage in PC12 cells.Methods: Oxidative stress damage of PC12 cells was induced by H2O2 at 200 μmol·L-1.The survival rate and damage degree were determined by MTT reduction assay and lactate dehydrogenase(LDH) release.Intracellular reactive oxygen species(ROS) generation and the changes in mitochondrial membrane potential were detected by fluorescent probe DCFH-DA and JC-1,respectively.Results: Compared with the normal control,H2O2 reduced the cell viability and depressed mitochondrial membrane potential,whereas increased LDH release and ROS production.Pretreatment with DL(10-7~10-5 mol·L-1) attenuated viability reduction,depression of mitochondrial membrane potential,LDH release and ROS generation induced by H2O2.Conclusion: DL protects PC12 cells against H2O2-induced oxidative stress damage and its mechanism may be related to ROS decrease and stability of mitochondrial membrane potential.