The Effect of Carnosine on Collagen Content in Rat Cardiac Fibroblast Under High Glucose Condition

Objective: To investigate the effect and mechanism of carnosine on collagen content in rat cardiac fibroblasts under high glucose condition.Methods: The rat cardiac fibroblasts were divided into seven groups,NG group(5.5mmol/L glucose),HG group(25mmol/L glucose),HG+10mmol/L carnosine group,HG+20mmol/L carnosine group,HG+40mmol/L carnosine group,HG+SB group(HG+10 μmol/LSB203580),HG+PD group(HG+10 μmol/L PD98059).The collagenⅠand Ⅲ contents were detected by ELISA,and Western blotting was used to detect the protein expression level of TGF-β1,p-p38 MAPK and p-ERK(1/2).Results: By the end of the experiment,compared with NG group,the expression level of collagen Ⅰand collagen Ⅲ were significantly higher in the HG group(P0.01);The protein expression level of TGF-β1,p-p38,p-ERK were also significantly higher in the HG group(P0.01).However,after supplement the carnosine,the level of collagen Ⅰ,collagen Ⅲ,TGF-β1,p-p38 and p-ERK were significantly reduced in HG+Car group than HG group(P0.05);Compared with HG group,the collagen Ⅰand Ⅲ were also significantly decreased in HG+SB group and HG+PD group.Conclusion: Carnosine reduced collagen production under high glucose via MAPK signaling pathway.