Somatic embryogenesis and plant regeneration from Carya cathayensis embryos using different carbon sources

A process for studying somatic embryogenesis and plant regeneration in vitro from immature embryos of Carya cathayensis,which were 10-12 weeks post-pollination,was developed.To select a carbon source(glucose,sucrose,trehalose,and maltose) for embryonic callus,a single-factor experimental design was used.Then,the optimal concentration of glucose(10,20,40,60 and 80 g·L-1) was determined.Also,for somatic embryogenesis,the best carbon source was decided.Then,the optimal sucrose concentrations(15,30,45,60 and 75 g·L-1) for somatic embryogenesis and for somatic embryo germination were determined.Results showed that the embryogenic callus induction rate of immature embryos inoculated in the medium supplemented with glucose was significantly higher(P 0.05) than sucrose,trehalose,and maltose.Embryogenic callus percentage was significantly greater(P 0.05) with 20 g·L-1 glucose with a 50.0%.For somatic embryogenesis of immature embryos,sucrose as a carbon source was significantly better(P 0.05) than glucose,trehalose,and maltose.Somatic embryogenesis induction was significantly higher(P 0.05) with 45 g·L-1 sucrose.For somatic embryo germination,a basal woody plant medium(WPM) supplemented with 5 g·L-1 sucrose was significantly higher(P 0.05) than other concentrations.