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2007•Acta Horticulturae SinicaRequires access

The RAPD Markers Linked to Downy Mildew Resistant Gene in Non-heading Chinese Cabbage (Brassica campestris ssp.chinensis Makino)

Hou XiLin

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Abstract

The objective of this study was to identify RAPD(Random Amplified Polymorphic DNA) markers for downy mildew resistance in non-heading Chinese cabbage.RAPD-PCR(Polymerase Chain Reaction) analysis was conducted using BSA(Bulked Segregant Analysis) of a segregating F2 population which has 144 individuals from a cross between 010(susceptible) and 014(resistant).After performing RAPD-PCR analysis with 560 arbitrary 10-mer primers and agarose-gel electrophoresis,only one RAPD marker AY121238 was identified to be linked to the resistant gene and was determined to be 6.7 cM from the resistant gene.

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What this paper is about

The objective of this study was to identify RAPD(Random Amplified Polymorphic DNA) markers for downy mildew resistance in non-heading Chinese cabbage.RAPD-PCR(Polymerase Chain Reaction) analysis was conducted using BSA(Bulked Segregant Analysis) of a segregating F2 population which has 144 individuals from a cross between 010(susceptible) and 014(resistant).After performing RAPD-PCR analysis with 560 arbitrary 10-mer primers and agarose-gel electrophoresis,only one RAPD marker AY121238 was identified to be linked to the resistant gene and was determined to be 6.7 cM from the resistant gene.

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Available abstract

The objective of this study was to identify RAPD(Random Amplified Polymorphic DNA) markers for downy mildew resistance in non-heading Chinese cabbage.RAPD-PCR(Polymerase Chain Reaction) analysis was conducted using BSA(Bulked Segregant Analysis) of a segregating F2 population which has 144 individuals from a cross between 010(susceptible) and 014(resistant).After performing RAPD-PCR analysis with 560 arbitrary 10-mer primers and agarose-gel electrophoresis,only one RAPD marker AY121238 was identified to be linked to the resistant gene and was determined to be 6.7 cM from the resistant gene.

Key concepts: RAPD, Downy mildew, Bulked segregant analysis, Biology, Genetics, Brassica, Population, Gene

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The RAPD Markers Linked to Downy Mildew Resistant Gene in Non-heading Chinese Cabbage (Brassica campestris ssp.chinensis Makino) — Research Paper | ScholarLens