ScholarLens Back to search
2012•Journal of Henan Normal UniversityRequires access

Construction of the Expression Stain of Human Interleukin-15

Chong Xu

Open publisher page 0 citations

Abstract

Total RNA is prepared from Monocytes which are obtained from healthy volunteers.Human interleukin-15(IL-15) gene is amplified with RT-PCR.The recombinant plasmid(pET28c(+)-IL-15) containing IL-15 gene is obtained and transformed into Escherichia coli BL21(DE3).The recombinant plasmid(pET28c(+)-IL-15) contains the gene IL-15.The result of Western blot shows that the expression protein is correct and expressed high in Escherichia coli.The expression level of the IL-15 protein is about 32% of total cellular protein.This lays the foundation for study of its function and clinical application.

About this research paper

What this paper is about

Total RNA is prepared from Monocytes which are obtained from healthy volunteers.Human interleukin-15(IL-15) gene is amplified with RT-PCR.The recombinant plasmid(pET28c(+)-IL-15) containing IL-15 gene is obtained and transformed into Escherichia coli BL21(DE3).The recombinant plasmid(pET28c(+)-IL-15) contains the gene IL-15.The result of Western blot shows that the expression protein is correct and expressed high in Escherichia coli.The expression level of the IL-15 protein is about 32% of total cellular protein.This lays the foundation for study of its function and clinical application.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Total RNA is prepared from Monocytes which are obtained from healthy volunteers.Human interleukin-15(IL-15) gene is amplified with RT-PCR.The recombinant plasmid(pET28c(+)-IL-15) containing IL-15 gene is obtained and transformed into Escherichia coli BL21(DE3).The recombinant plasmid(pET28c(+)-IL-15) contains the gene IL-15.The result of Western blot shows that the expression protein is correct and expressed high in Escherichia coli.The expression level of the IL-15 protein is about 32% of total cellular protein.This lays the foundation for study of its function and clinical application.

Key concepts: Recombinant DNA, Molecular biology, Escherichia coli, Plasmid, Gene expression, Gene, Western blot, Biology

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Construction of the Expression Stain of Human Interleukin-15 — Research Paper | ScholarLens